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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
19/06/2015 |
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Data da última atualização: |
11/05/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
AZEVEDO, H.; LOPES, F. M.; SILLA, P. R.; HUNGRIA, M. |
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Afiliação: |
HELTON DE AZEVEDO, CNPSO; Fabricio Martins Lopes; PAULO ROBERTO SILLA, CNPSO; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
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Título: |
A database for the taxonomic and phylogenetic identification of the genus Bradyrhizobium using multilocus sequence analysis. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
BMC Genomics, London, v. 16, 2015. Suppl 5, S10. |
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Páginas: |
11 p. |
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ISSN: |
1471-2164 |
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Idioma: |
Inglês |
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Notas: |
Edição dos Proceedings of the 10th International Conference of the Brazilian Association for Bioinformatics and Computational Biology (X-Meeting 2014), Belo Horizonte, Oct. 2014. |
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Conteúdo: |
Biological nitrogen fixation, with an emphasis on the legume-rhizobia symbiosis, is a key process for agriculture and the environment, allowing the replacement of nitrogen fertilizers, reducing water pollution by nitrate as well as emission of greenhouse gases. Soils contain numerous strains belonging to the bacterial genus Bradyrhizobium, which establish symbioses with a variety of legumes. However, due to the high conservation of Bradyrhizobium 16S rRNA genes - considered as the backbone of the taxonomy of prokaryotes - few species have been delineated. The multilocus sequence analysis (MLSA) methodology, which includes analysis of housekeeping genes, has been shown to be promising and powerful for defining bacterial species, and, in this study, it was applied to Bradyrhizobium, species, increasing our understanding of the diversity of nitrogen-fixing bacteria. Description: Classification of bacteria of agronomic importance is relevant to biodiversity, as well as to biotechnological manipulation to improve agricultural productivity. We propose the construction of an online database that will provide information and tools using MLSA to improve phylogenetic and taxonomic characterization of Bradyrhizobium, allowing the comparison of genomic sequences with those of type and representative strains of each species. Conclusion: A database for the taxonomic and phylogenetic identification of the Bradyrhizobium, genus, using MLSA, will facilitate the use of biological data available through an intuitive web interface. Sequences stored in the on-line database can be compared with multiple sequences of other strains with simplicity and agility through multiple alignment algorithms and computational routines integrated into the database. The proposed database and software tools are available at http://mlsa.cnpso.embrapa.br, and can be used, free of charge, by researchers worldwide to classify Bradyrhizobium, strains; the database and software can be applied to replicate the experiments presented in this study as well as to generate new experiments. The next step will be expansion of the database to include other rhizobial species. MenosBiological nitrogen fixation, with an emphasis on the legume-rhizobia symbiosis, is a key process for agriculture and the environment, allowing the replacement of nitrogen fertilizers, reducing water pollution by nitrate as well as emission of greenhouse gases. Soils contain numerous strains belonging to the bacterial genus Bradyrhizobium, which establish symbioses with a variety of legumes. However, due to the high conservation of Bradyrhizobium 16S rRNA genes - considered as the backbone of the taxonomy of prokaryotes - few species have been delineated. The multilocus sequence analysis (MLSA) methodology, which includes analysis of housekeeping genes, has been shown to be promising and powerful for defining bacterial species, and, in this study, it was applied to Bradyrhizobium, species, increasing our understanding of the diversity of nitrogen-fixing bacteria. Description: Classification of bacteria of agronomic importance is relevant to biodiversity, as well as to biotechnological manipulation to improve agricultural productivity. We propose the construction of an online database that will provide information and tools using MLSA to improve phylogenetic and taxonomic characterization of Bradyrhizobium, allowing the comparison of genomic sequences with those of type and representative strains of each species. Conclusion: A database for the taxonomic and phylogenetic identification of the Bradyrhizobium, genus, using MLSA, will facilitate the use of biological data availab... Mostrar Tudo |
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Thesagro: |
Fixação de nitrogênio. |
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Thesaurus Nal: |
Nitrogen fixation. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/125643/1/A-database-for-the-taxonomic-and-phylogenetic.pdf
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Marc: |
LEADER 02982naa a2200217 a 4500
001 2018160
005 2017-05-11
008 2015 bl uuuu u00u1 u #d
022 $a1471-2164
100 1 $aAZEVEDO, H.
245 $aA database for the taxonomic and phylogenetic identification of the genus Bradyrhizobium using multilocus sequence analysis.$h[electronic resource]
260 $c2015
300 $a11 p.
500 $aEdição dos Proceedings of the 10th International Conference of the Brazilian Association for Bioinformatics and Computational Biology (X-Meeting 2014), Belo Horizonte, Oct. 2014.
520 $aBiological nitrogen fixation, with an emphasis on the legume-rhizobia symbiosis, is a key process for agriculture and the environment, allowing the replacement of nitrogen fertilizers, reducing water pollution by nitrate as well as emission of greenhouse gases. Soils contain numerous strains belonging to the bacterial genus Bradyrhizobium, which establish symbioses with a variety of legumes. However, due to the high conservation of Bradyrhizobium 16S rRNA genes - considered as the backbone of the taxonomy of prokaryotes - few species have been delineated. The multilocus sequence analysis (MLSA) methodology, which includes analysis of housekeeping genes, has been shown to be promising and powerful for defining bacterial species, and, in this study, it was applied to Bradyrhizobium, species, increasing our understanding of the diversity of nitrogen-fixing bacteria. Description: Classification of bacteria of agronomic importance is relevant to biodiversity, as well as to biotechnological manipulation to improve agricultural productivity. We propose the construction of an online database that will provide information and tools using MLSA to improve phylogenetic and taxonomic characterization of Bradyrhizobium, allowing the comparison of genomic sequences with those of type and representative strains of each species. Conclusion: A database for the taxonomic and phylogenetic identification of the Bradyrhizobium, genus, using MLSA, will facilitate the use of biological data available through an intuitive web interface. Sequences stored in the on-line database can be compared with multiple sequences of other strains with simplicity and agility through multiple alignment algorithms and computational routines integrated into the database. The proposed database and software tools are available at http://mlsa.cnpso.embrapa.br, and can be used, free of charge, by researchers worldwide to classify Bradyrhizobium, strains; the database and software can be applied to replicate the experiments presented in this study as well as to generate new experiments. The next step will be expansion of the database to include other rhizobial species.
650 $aNitrogen fixation
650 $aFixação de nitrogênio
700 1 $aLOPES, F. M.
700 1 $aSILLA, P. R.
700 1 $aHUNGRIA, M.
773 $tBMC Genomics, London$gv. 16, 2015. Suppl 5, S10.
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Registro original: |
Embrapa Soja (CNPSO) |
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Biblioteca |
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| Registros recuperados : 11 | |
| 1. |  | AZEVEDO, H.; LOPES, F. M.; SILLA, P. R.; HUNGRIA, M. A database for the taxonomic and phylogenetic identification of the genus Bradyrhizobium using multilocus sequence analysis. BMC Genomics, London, v. 16, 2015. Suppl 5, S10. 11 p. Edição dos Proceedings of the 10th International Conference of the Brazilian Association for Bioinformatics and Computational Biology (X-Meeting 2014), Belo Horizonte, Oct. 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Soja. |
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| 5. | | PACANHELA, E. F.; RONDINA, A. B. L.; NOGUEIRA, M. A.; HUNGRIA, M.; SAITO, P. T. M.; LOPES, F. M. Análise e Classificação Automática de Nódulos em Raízes de Cultivares de Soja. In: BRAZILIAN E-SCIENCE WORKSHOP (BRESCI), 16., 2022, Niterói. Anais [...]. Porto Alegre: SBC, 2022. p. 41-48.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 6. | | SILVA, C. N. da; FALEIRO, F. G.; JUNQUEIRA, N. T. V.; COSTA, A. M.; LOPES, F. M. Caracterização morfoagronômica e ganhos de seleção em progênies de meio-irmãos de Passiflora maliformis L. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA CERRADOS, 2015, Planaltina, DF. Jovens Talentos 2015: resumos. Planaltina, DF: Embrapa Cerrados, 2015. (Embrapa Cerrados. Documentos, 328). p. 29| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Cerrados. |
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| 7. | | D'ABADIA, A. C. A.; FALEIRO, F. G.; COSTA, A. M.; JUNQUEIRA, N. T. V.; BRAGA, M. F.; LOPES, F. M. Características físicas de frutos de maracujazeiro-doce sob diferentes sistemas de condução. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA CERRADOS, 2015, Planaltina, DF. Jovens Talentos 2015: resumos. Planaltina, DF: Embrapa Cerrados, 2015. (Embrapa Cerrados. Documentos, 328). p. 25| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Cerrados. |
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| 9. | | PASCHOAL, A. R.; FERNANDES, E. D. M.; SILVA, J. C.; LOPES, F. M.; PEREIRA, L. F. P.; DOMINGUES, D. S. CoffeebEST: an integrated resource for Coffea spp expressed sequence tags. Genetics and Molecular Research, v. 13, n. 4, p. 10913-10920, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Café. |
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| 10. | | SILVA, C. N. da; FALEIRO, F. G.; JUNQUEIRA, N. T. V.; COSTA, A. M.; LOPES, F. M. Diferencial de seleção em progênies de passiflora maliformis. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 8., 2015, Goiânia. O melhoramento de plantas, o futuro da agricultura e a soberania nacional: anais. Goiânia: UFG: SBMP, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Cerrados. |
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| 11. | | DABADIA, A. C. A.; FALEIRO, F. G.; COSTA, A. M.; JUNQUEIRA, N. T. V.; BRAGA, M. F.; LOPES, F. M. Variabilidade genética e características físicas de frutos de matrizes selecionadas de maracujazeiro doce. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 8., 2015, Goiânia. O melhoramento de plantas, o futuro da agricultura e a soberania nacional: anais. Goiânia: UFG: SBMP, 2015.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Cerrados. |
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| Registros recuperados : 11 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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